GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12376003
| Citation |
Proux-Gillardeaux, V, Galli, T, Callebaut, I, Mikhailik, A, Calothy, G and Marx, M (2003) D53 is a novel endosomal SNARE-binding protein that enhances interaction of syntaxin 1 with the synaptobrevin 2 complex in vitro. Biochem. J. 370:213-21 |
|---|---|
| Abstract |
Synaptobrevin 2 (Sb2), syntaxin1 (Stx1), and synaptosomal-associated protein of 25 kDa (SNAP-25) are the main components of the soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex involved in fusion of synaptic vesicles with the presynaptic plasma membrane. We report the characterization of D53, a novel SNARE-binding protein preferentially expressed in neural and neuro-endocrine cells. Its two-dimensional organization, established by the hydrophobic cluster analysis, is reminiscent of SNARE proteins. D53 contains two putative helical regions, one of which includes a large coiled-coil domain involved in the interaction with Sb2 in vitro. Following subcellular fractionation, endogenous D53 was specifically detected in the membrane-containing fraction of PC12 cells, where it co-immunoprecipitated with Sb2. Analysis by confocal microscopy showed that, in these cells, endogenous D53 co-localized partially with the transferrin receptor in early endosomes. In vitro assays revealed that binding properties of D53 to Stx1 and Sb2 are comparable with those of SNAP-25. Furthermore, D53 forms Sb2/Stx1/D53 complexes in vitro in a manner similar to SNAP-25. We propose that D53 could be involved in the assembly or disassembly of endosomal SNARE complexes by regulating Sb2/Stx interaction. |
| Links |
PubMed PMC1223131 Online version:10.1042/BJ20021309 |
| Keywords |
Amino Acid Sequence; Animals; Antigens, Surface/metabolism; COS Cells; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Endosomes/metabolism; Fluorescent Antibody Technique; HeLa Cells; Humans; Membrane Proteins/metabolism; Molecular Sequence Data; Mutagenesis; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; PC12 Cells; Protein Binding; R-SNARE Proteins; Rats; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; SNARE Proteins; Sequence Homology, Amino Acid; Syntaxin 1; Vesicular Transport Proteins |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
