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PMID:12200151
| Citation |
Yuan, SS, Yeh, YT and Lee, EY (2002) Pax-2 interacts with RB and reverses its repression on the promoter of Rig-1, a Robo member. Biochem. Biophys. Res. Commun. 296:1019-25 |
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| Abstract |
RB plays dual roles in the regulation of cell proliferation and differentiation. The nervous tissue-specific gene Rig-1, a member of the roundabout (Robo) guidance receptor family, was identified as an RB-regulated gene in the mouse embryo. Herein, we report that a 2.3kb genomic DNA fragment, which contains the first 129 bases of the 5'-untranslated region and 2.2kb of the 5'-flanking region of Rig-1, has a cell type-specific promoter activity. Rig-1 promoter activity is downregulated by RB and upregulated by Pax-2. Furthermore, Rig-1 and Pax-2 mRNAs are coexpressed in the hindbrain and spinal cord of the E11.5 mouse embryo, suggesting that Pax-2 may regulate Rig-1 expression during the embryonic stage. Pax-2 interacts with RB and reverses its transcriptional suppression on the Rig-1 promoter. In summary, the ubiquitous tumor suppressor RB and the neuron-enriched transcription factor Pax-2 may play a role in the regulation of Rig-1 expression during embryogenesis. |
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| Keywords |
3T3 Cells; 5' Untranslated Regions; Animals; Brain/metabolism; DNA/metabolism; DNA, Complementary/metabolism; DNA-Binding Proteins/metabolism; Down-Regulation; Genes, Reporter; Immunoblotting; In Situ Hybridization; Luciferases/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Molecular Sequence Data; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; PAX2 Transcription Factor; Precipitin Tests; Promoter Regions, Genetic; Protein Binding; RNA, Messenger/metabolism; Receptors, Immunologic/genetics; Retinoblastoma Protein/metabolism; Spinal Cord/metabolism; Time Factors; Transcription Factors/metabolism; Transcription, Genetic; Transfection; Up-Regulation |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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