GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12176133
| Citation |
Cai, S, Alp, NJ, McDonald, D, Smith, I, Kay, J, Canevari, L, Heales, S and Channon, KM (2002) GTP cyclohydrolase I gene transfer augments intracellular tetrahydrobiopterin in human endothelial cells: effects on nitric oxide synthase activity, protein levels and dimerisation. Cardiovasc. Res. 55:838-49 |
|---|---|
| Abstract |
Tetrahydrobiopterin (BH4) is an essential cofactor for endothelial nitric oxide synthase (eNOS) activity. BH4 levels are regulated by de novo biosynthesis; the rate-limiting enzyme is GTP cyclohydrolase I (GTPCH). BH4 activates and promotes homodimerisation of purified eNOS protein, but the intracellular mechanisms underlying BH4-mediated eNOS regulation in endothelial cells remain less clear. We aimed to investigate the role of BH4 levels in intracellular eNOS regulation, by targeting the BH4 synthetic pathway as a novel strategy to modulate intracellular BH4 levels. |
| Links | |
| Keywords |
3T3 Cells; Adenoviridae/genetics; Animals; Biopterin/analogs & derivatives; Biopterin/metabolism; Cell Line; Dimerization; Endothelium, Vascular/metabolism; GTP Cyclohydrolase/genetics; GTP Cyclohydrolase/metabolism; Genetic Vectors/genetics; Humans; Intracellular Fluid/metabolism; Mice; Nitric Oxide Synthase/metabolism; Transfection/methods |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
