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PMID:12176032
| Citation |
Chen, XQ and Yu, AC (2002) The association of 14-3-3gamma and actin plays a role in cell division and apoptosis in astrocytes. Biochem. Biophys. Res. Commun. 296:657-63 |
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| Abstract |
The 14-3-3 protein family plays critical regulatory roles in signaling pathways in cell division and apoptosis. 14-3-3gamma is mainly expressed in brain. Using primary cultures of cerebral cortical astrocytes, we investigated the relationships between 14-3-3gamma proteins and actin in astrocytes in cell division and under ischemia. Our results showed that endogenous 14-3-3gamma proteins in immature astrocytes appeared filamentous and co-localized with filamentous actin (F-actin). During certain stages of mitosis, 14-3-3gamma proteins first aggregated and then formed a ring-like structure that surrounded the daughter nuclei and enclosed the F-actin. In 4-week-old cultures of astrocytes, 14-3-3gamma proteins appeared as punctate aggregates in the cytoplasm. Under ischemia, 14-3-3gamma proteins formed filamentous structures and were closely associated with F-actin in surviving astrocytes. However, in apoptotic astrocytes, the intensity of immunostaining of 14-3-3gamma proteins in the cytoplasm decreased. The proteins aggregated around the nucleus and dissociated from the actin filaments. Reciprocal co-immunoprecipitations demonstrated that endogenous 14-3-3gamma proteins bound to detergent-soluble actin and the level of binding increased after 4h of ischemia. As actin is a critical structural protein heavily involved in cell division and apoptotic death, our findings suggest that 14-3-3gamma proteins play a role in cytoskeletal function during the process of cell division and apoptosis in astrocytes in association with actin. |
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| Keywords |
14-3-3 Proteins; Actin Cytoskeleton/ultrastructure; Actins/analysis; Actins/metabolism; Actins/physiology; Animals; Animals, Newborn; Apoptosis; Astrocytes/cytology; Astrocytes/metabolism; Astrocytes/ultrastructure; Cell Division; Cell Hypoxia; Cells, Cultured; Mice; Mice, Inbred ICR; Mitosis; Tyrosine 3-Monooxygenase/analysis; Tyrosine 3-Monooxygenase/metabolism; Tyrosine 3-Monooxygenase/physiology |
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Significance
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