PMID:12164808

Citation	Seki, M, Narusaka, M, Ishida, J, Nanjo, T, Fujita, M, Oono, Y, Kamiya, A, Nakajima, M, Enju, A, Sakurai, T, Satou, M, Akiyama, K, Taji, T, Yamaguchi-Shinozaki, K, Carninci, P, Kawai, J, Hayashizaki, Y and Shinozaki, K (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J. 31:279-92
Abstract	Full-length cDNAs are essential for functional analysis of plant genes in the post-sequencing era of the Arabidopsis genome. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. We have prepared a full-length cDNA microarray containing approximately 7000 independent, full-length cDNA groups to analyse the expression profiles of genes under drought, cold (low temperature) and high-salinity stress conditions over time. The transcripts of 53, 277 and 194 genes increased after cold, drought and high-salinity treatments, respectively, more than fivefold compared with the control genes. We also identified many highly drought-, cold- or high-salinity- stress-inducible genes. However, we observed strong relationships in the expression of these stress-responsive genes based on Venn diagram analysis, and found 22 stress-inducible genes that responded to all three stresses. Several gene groups showing different expression profiles were identified by analysis of their expression patterns during stress-responsive gene induction. The cold-inducible genes were classified into at least two gene groups from their expression profiles. DREB1A was included in a group whose expression peaked at 2 h after cold treatment. Among the drought, cold or high-salinity stress-inducible genes identified, we found 40 transcription factor genes (corresponding to approximately 11% of all stress-inducible genes identified), suggesting that various transcriptional regulatory mechanisms function in the drought, cold or high-salinity stress signal transduction pathways.
Links	PubMed
Keywords	Arabidopsis/drug effects; Arabidopsis/genetics; Cold Temperature; Desiccation; Disasters; Gene Expression Profiling; Gene Expression Regulation, Plant/drug effects; Genes, Plant/genetics; Oligonucleotide Array Sequence Analysis; Plant Diseases/chemically induced; Plant Diseases/genetics; Promoter Regions, Genetic/genetics; RNA, Plant/analysis; RNA, Plant/genetics; Sodium Chloride/pharmacology; Time Factors; Transcriptional Activation
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