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PMID:12119130
Citation |
Sakazaki, H, Ueno, H and Nakamuro, K (2002) Estrogen receptor alpha in mouse splenic lymphocytes: possible involvement in immunity. Toxicol. Lett. 133:221-9 |
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Abstract |
To elucidate relevance of estrogens to immune responses, we investigated whether estrogen receptor alpha (ERalpha) exists in mouse splenic B cells and T cells and the effect of 17beta-estradiol and endocrine disrupting chemicals (EDCs) on lymphocyte mitogenesis. ERalpha was identified in both male and female mouse splenic cells using RT-PCR. Crude splenic cells were stained with anti-ER antibody, and the distribution of ERalpha in the splenic B cells and part of the splenic T cells was confirmed by flow cytometry. 17beta-Estradiol inhibited B cell mitogenesis at the concentration of 10(-8) M and T cell mitogenesis at the concentration of 10(-6) M. Some EDCs, diethylstilbestrol, bisphenol A, p-nonylphenol and di-2-ethylhexylphthalate, suppressed lymphocyte mitogenesis at the concentration of 10(-6)-10(-5) M. We therefore suggest that estrogen may suppress lymphocyte mitogenesis through ERalpha in B and T cells. |
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Keywords |
Animals; Bone Marrow Cells/drug effects; Cell Division/drug effects; Environmental Pollutants/toxicity; Estradiol/toxicity; Estrogen Receptor alpha; Estrogens/toxicity; Estrogens, Non-Steroidal/toxicity; Female; Flow Cytometry; Lymphocytes/immunology; Lymphocytes/metabolism; Male; Mice; Mice, Inbred BALB C; RNA/biosynthesis; RNA/isolation & purification; Receptors, Estrogen/immunology; Receptors, Estrogen/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Spleen/immunology; Spleen/metabolism |
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