GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:12117804

From GONUTS
Jump to: navigation, search
Citation

Saunders, CM, Larman, MG, Parrington, J, Cox, LJ, Royse, J, Blayney, LM, Swann, K and Lai, FA (2002) PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development. Development 129:3533-44

Abstract

Upon fertilisation by sperm, mammalian eggs are activated by a series of intracellular Ca(2+) oscillations that are essential for embryo development. The mechanism by which sperm induces this complex signalling phenomenon is unknown. One proposal is that the sperm introduces an exclusive cytosolic factor into the egg that elicits serial Ca(2+) release. The 'sperm factor' hypothesis has not been ratified because a sperm-specific protein that generates repetitive Ca(2+) transients and egg activation has not been found. We identify a novel, sperm-specific phospholipase C, PLC zeta, that triggers Ca(2+) oscillations in mouse eggs indistinguishable from those at fertilisation. PLC zeta removal from sperm extracts abolishes Ca(2+) release in eggs. Moreover, the PLC zeta content of a single sperm was sufficient to produce Ca(2+) oscillations as well as normal embryo development to blastocyst. Our results are consistent with sperm PLC zeta as the molecular trigger for development of a fertilised egg into an embryo.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Benzofurans/metabolism; Calcium Signaling/physiology; Cloning, Molecular; Embryonic and Fetal Development; Fertilization/physiology; Fluorescent Dyes/metabolism; Imidazoles/metabolism; Isoenzymes/chemistry; Isoenzymes/genetics; Isoenzymes/metabolism; Male; Mice; Microinjections; Molecular Sequence Data; Ovum/metabolism; Phosphoinositide Phospholipase C; Phylogeny; Sequence Alignment; Spermatozoa/enzymology; Tissue Distribution; Type C Phospholipases/chemistry; Type C Phospholipases/classification; Type C Phospholipases/genetics; Type C Phospholipases/metabolism

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.