GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12093753
| Citation |
Jimeno, S, Rondón, AG, Luna, R and Aguilera, A (2002) The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability. EMBO J. 21:3526-35 |
|---|---|
| Abstract |
The THO complex is a multimeric factor containing four polypeptides, Tho2, Hpr1, Mft1 and Thp2. Mutations in any of the genes encoding THO confer impairment of transcription and a transcription-dependent hyper-recombination phenotype, suggesting that THO has a functional role in gene expression. Using an in vivo assay developed to study expression of long and G+C-rich DNA sequences, we have isolated SUB2, a gene involved in mRNA splicing and export, as a multicopy suppressor of the gene expression defect of hpr1 Delta. Further investigation of a putative functional relationship between mRNA metabolism and THO revealed that mRNA export mutants sub2, yra1, mex67 and mtr2 have similar defective transcription and hyper-recombination phenotypes as THO mutants. In addition, THO becomes essential in cells with a defective Mex67 mRNA export er. Finally, we have shown that THO has the ability to associate with RNA and DNA in vitro. These results indicate a functional link between the processes of elongation and metabolism of nascent mRNA mediated by THO and mRNA export proteins, which have important consequences for the maintenance of genome stability. |
| Links |
PubMed PMC126085 Online version:10.1093/emboj/cdf335 |
| Keywords |
Biological Transport; DNA, Fungal/genetics; DNA, Fungal/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Gene Expression Regulation, Fungal; Macromolecular Substances; Models, Biological; Nuclear Proteins/genetics; Nuclear Proteins/physiology; Nucleocytoplasmic Transport Proteins; RNA Helicases/deficiency; RNA Helicases/genetics; RNA Helicases/physiology; RNA, Fungal/metabolism; RNA, Messenger/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/physiology; Recombination, Genetic; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/physiology; Transcription Factors/genetics; Transcription Factors/physiology; Transcription, Genetic |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
