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PMID:12061771
| Citation |
Nagai, H, Oshiro, N, Takuwa-Kuroda, K, Iwanaga, S, Nozaki, M and Nakajima, T (2002) Novel proteinaceous toxins from the nematocyst venom of the Okinawan sea anemone Phyllodiscus semoni Kwietniewski. Biochem. Biophys. Res. Commun. 294:760-3 |
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| Abstract |
The Okinawan sea anemone Phyllodiscus semoni is known to cause cases of severe stinging. We isolated P. semoni toxins 60A and 60B (PsTX-60A and PsTX-60B; ca. 60 kDa) as the major toxins from the isolated nematocysts of this species for the first time. PsTX-60A and PsTX-60B showed lethal toxicity to the shrimp Palaemon paucidence when administered via intraperitoneal injection (LD(50) values: 800-900 and 800 microg/kg, respectively) and hemolytic activity toward a 0.8% suspension of sheep red blood cells (ED(50) values: 600 and 300 ng/ml, respectively). Furthermore, we sequenced the cDNA encoding PsTX-60A. The deduced amino acid sequence of PsTX-60A did not show any similarity to previously reported proteins. The N-terminal amino acid sequence of PsTX-60B showed homology with that of PsTX-60A. These toxins represent a novel class of cytolytic proteinaceous toxins. |
| Links |
PubMed Online version:10.1016/S0006-291X(02)00547-8 |
| Keywords |
Algorithms; Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA, Complementary/metabolism; Decapoda (Crustacea); Erythrocytes/drug effects; Marine Toxins/chemistry; Marine Toxins/isolation & purification; Marine Toxins/toxicity; Molecular Sequence Data; Protein Biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; Sea Anemones; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sheep |
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Significance
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