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PMID:12056833
| Citation |
Kappler, J, Baader, SL, Franken, S, Pesheva, P, Schilling, K, Rauch, U and Gieselmann, V (2002) Tenascins are associated with lipid rafts isolated from mouse brain. Biochem. Biophys. Res. Commun. 294:742-7 |
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| Abstract |
Lipid rafts are microdomains of the plasma membrane which are enriched in glycosphingolipids and specific proteins. The reported interactions of several raft-associated proteins (such as, e.g., F3) with tenascin C and tenascin R prompted us to consider that these oligomeric multidomain glycoproteins of the extracellular matrix (ECM) could associate with rafts. Here, we show punctate immunocytochemical distributions of tenascin C (TN-C) and tenascin R (TN-R) at the membrane surface of neural cells resembling the pattern reported for raft-associated proteins. Moreover, cholesterol depletion with methyl-beta-cyclodextrin reduced the punctate surface staining of TN-C. Consistently, TN-C was associated with lipid rafts of neonatal mouse brain according to sucrose density gradient centrifugation experiments. Furthermore, TN-R was also found in rafts prepared from myelin of adult mice. Thus, brain-derived tenascins are able to associate with lipid rafts. |
| Links |
PubMed Online version:10.1016/S0006-291X(02)00520-X |
| Keywords |
Animals; Brain Chemistry; Cell Adhesion Molecules/metabolism; Cells, Cultured; Female; Membrane Microdomains/metabolism; Mice; Neurons/metabolism; Neurons/ultrastructure; Pregnancy; Tenascin/metabolism |
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Significance
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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