GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12054664
| Citation |
Matsumoto, M, Kikkawa, S, Kohase, M, Miyake, K and Seya, T (2002) Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293:1364-9 |
|---|---|
| Abstract |
A monoclonal antibody (mAb) against human Toll-like receptor (TLR) 3 was established and its effect on TLR3-mediated responses was tested using human fibroblast cell lines expressing TLR3 on the cell surface. Fibroblasts are known to produce IFN-beta upon viral infection or treatment with double-stranded RNA (dsRNA) through distinct signaling pathways. Here, we show the mAb to TLR3 suppressed poly(I):poly(C)-mediated IFN-beta production by human fibroblasts naturally expressing TLR3 on their surface. By reporter gene assay using HEK293 cells transfected with a human TLR3 expression vector, TLR3 recognized dsRNA to activate NF-kappaB and the IFN-beta promoter. TLR3 signaling was not elicited by either single-stranded RNA (ssRNA) or dsDNA. Thus, specific recognition of dsRNA by extracellular TLR3 is essential for induction of type I IFN: the interassociation between dsRNA and TLR3, regardless of direct or indirect binding, should be disrupted by mAb being attached to TLR3. The mAb against TLR3 reported herein may serve as a regulator for virus-mediated immune response via an alternative pathway involving the dsRNA-TLR3 recognition which might occur on host cells. |
| Links |
PubMed Online version:10.1016/S0006-291X(02)00380-7 |
| Keywords |
Animals; Antibodies, Monoclonal/metabolism; Cell Line; Cell Membrane/metabolism; DNA, Complementary/metabolism; Dose-Response Relationship, Drug; Drosophila Proteins; Fibroblasts/metabolism; Flow Cytometry; Genes, Reporter; Genetic Vectors; Humans; Interferon-beta/metabolism; Luciferases/metabolism; Lung/metabolism; Membrane Glycoproteins/chemistry; Membrane Glycoproteins/immunology; Mice; Mice, Inbred BALB C; NF-kappa B/metabolism; Promoter Regions, Genetic; Protein Binding; RNA/metabolism; RNA, Double-Stranded/metabolism; Receptors, Cell Surface/chemistry; Receptors, Cell Surface/immunology; Recombinant Proteins/metabolism; Signal Transduction; Toll-Like Receptor 3; Toll-Like Receptors; Transfection |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
