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PMID:12050152
| Citation |
Hu, J, Meng, Q, Roy, SK, Raha, A, Hu, J, Zhang, J, Hashimoto, K and Kalvakolanu, DV (2002) A novel transactivating factor that regulates interferon-gamma-dependent gene expression. J. Biol. Chem. 277:30253-63 |
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| Abstract |
We have previously identified a novel interferon (IFN)-stimulated cis-acting enhancer element, gamma-IFN-activated transcriptional element (GATE). GATE differs from the known IFN-stimulated elements in its primary sequence. Preliminary analysis has indicated that the GATE-dependent transcriptional response requires the binding of novel transacting factors. A cDNA expression library derived from an IFN-gamma-stimulated murine macrophage cell line was screened with a (32)P-labeled GATE probe to identify the potential GATE-binding factors. A cDNA coding for a novel transcription-activating factor was identified. Based on its discovery, we named it as GATE-binding factor-1 (GBF-1). GBF-1 homologs are present in mouse, human, monkey, and Drosophila. It activates transcription from reporter genes carrying GATE. It possesses a strong transactivating activity but has a weak DNA binding property. GBF-1 is expressed in most tissues with relatively higher steady-state levels in heart, liver, kidney, and brain. Its expression is induced by IFN-gamma treatment. GBF-1 is present in both cytosolic and nuclear compartments. These studies thus identify a novel transactivating factor in IFN signaling pathways. |
| Links |
PubMed Online version:10.1074/jbc.M202679200 |
| Keywords |
Amino Acid Sequence; Animals; Base Sequence; DNA Primers; DNA, Complementary; Gene Expression Regulation/physiology; Humans; Interferon-gamma/physiology; Intramolecular Oxidoreductases; Mice; Molecular Sequence Data; Sequence Homology, Amino Acid; Trans-Activators/chemistry; Trans-Activators/genetics; Trans-Activators/metabolism; Trans-Activators/physiology |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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