GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12021206
| Citation |
Lazarus, M, Munday, CJ, Eguchi, N, Matsumoto, S, Killian, GJ, Kubata, BK and Urade, Y (2002) Immunohistochemical localization of microsomal PGE synthase-1 and cyclooxygenases in male mouse reproductive organs. Endocrinology 143:2410-9 |
|---|---|
| Abstract |
We investigated the tissue distribution and cellular localization of microsomal PGE synthase-1 (mPGES-1) and cyclooxygenase (COX)-1 and -2 in the male mouse reproductive organs. Northern blotting revealed that the mPGES-1 mRNA was expressed intensely in the epididymis and weakly in the lung, spleen, skin, kidney, colon, and brain. In the male reproductive tract, the expression of mPGES-1 increased from the testis to the cauda epididymis and was highest in the vas deferens when examined by Northern blotting, RT-PCR, and Western blotting. By immunohistochemistry, mPGES-1 was detected in Leydig cells of the testis and in epithelial cells of the epididymis, vas deferens, and seminal vesicles. In addition, the caput and cauda regions of the epididymis and the vas deferens in this order showed a progressive increase in the expression of COX-1 mRNA and immunoreactivity, whereas COX-2 was dominantly expressed in the vas deferens. COX-1 was localized in epithelial cells of the caput, corpus and cauda epididymis and of the vas deferens, and COX-2 was evident in epithelial cells of the distal cauda epididymis and vas deferens. These results show that mPGES-1 is expressed coordinately with COX-1 and COX-2 and is involved in PGE(2) production in male genital organs. |
| Links | |
| Keywords |
Animals; Blotting, Northern; Blotting, Western; Cyclooxygenase 1; Cyclooxygenase 2; Dinoprostone/biosynthesis; Epididymis/metabolism; Epididymis/ultrasonography; Fluorescent Antibody Technique; Genitalia, Male/enzymology; Genitalia, Male/ultrastructure; Immunoenzyme Techniques; Immunohistochemistry; Intramolecular Oxidoreductases/metabolism; Isoenzymes/metabolism; Leydig Cells/metabolism; Leydig Cells/ultrastructure; Male; Membrane Proteins; Methyltransferases; Mice; Mice, Inbred C57BL; Microsomes/enzymology; Microsomes/ultrastructure; Prostaglandin-Endoperoxide Synthases/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Seminiferous Tubules/metabolism; Seminiferous Tubules/ultrastructure; Vas Deferens/metabolism; Vas Deferens/ultrasonography |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
