PMID:12015967

Citation	Audhya, A and Emr, SD (2002) Stt4 PI 4-kinase localizes to the plasma membrane and functions in the Pkc1-mediated MAP kinase cascade. Dev. Cell 2:593-605
Abstract	Production of the essential phospholipid PI4P at the Golgi by the Pik1 kinase is required for protein secretion, while a distinct pool of PI4P generated by the Stt4 kinase is critical for normal actin cytoskeleton organization. We identify a transmembrane protein that stabilizes Stt4 at the plasma membrane where it directs synthesis of PI4P, which is required for activation of the Rho1/Pkc1-mediated MAP kinase cascade. Inactivation of Stt4 or the PI4P 5-kinase Mss4 results in mislocalization of the Rho-GTPase GEF Rom2. Rom2 binds PI4,5P(2) through its PH domain and represents the first identified effector in the Stt4-Mss4 pathway. Based on these results, we propose that Stt4-Mss4 generates PI4,5P(2) at the plasma membrane, required to recruit/activate effector proteins such as Rom2.
Links	PubMed
Keywords	1-Phosphatidylinositol 4-Kinase/genetics; 1-Phosphatidylinositol 4-Kinase/metabolism; Cell Membrane/metabolism; Enzyme Activation; Fungal Proteins/metabolism; Genes, Fungal; Genes, Suppressor; Mitogen-Activated Protein Kinases/metabolism; Models, Biological; Phosphatidylinositol 4,5-Diphosphate; Phosphatidylinositol Phosphates/metabolism; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Protein Kinase C; Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism
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