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PMID:11988522
| Citation |
Elvers, KT and Park, SF (2002) Quorum sensing in Campylobacter jejuni: detection of a luxS encoded signalling molecule. Microbiology (Reading, Engl.) 148:1475-81 |
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| Abstract |
The expression of a wide variety of physiological functions in many bacterial species is modulated by quorum sensing, a population-dependent signalling mechanism that involves the production and detection of extracellular signalling molecules. The genome sequence of Campylobacter jejuni NCTC 11168 contains a gene encoding an orthologue of LuxS, which is required for autoinducer-2 (AI-2) production in other bacterial species, but does not contain genes predicted to encode any known acyl-homoserine lactone synthetase. This study demonstrates that C. jejuni produces functional AI-2 activity through the ability of cell-free extracts to specifically induce bioluminescence in Vibrio harveyi BB170, a reporter strain for quorum-sensing system 2. Production of this signalling compound was shown to be dependent upon the product of the C. jejuni luxS gene (Cj1198). While the luxS mutant showed comparable growth rate, resistance to oxidative stress and ability to invade Caco-2 cell monolayers to the parental strain, it exhibited decreased motility haloes in semisolid media, suggesting a role for quorum sensing in the regulation of motility. |
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| Keywords |
Amino Acid Sequence; Bacterial Adhesion; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Caco-2 Cells; Campylobacter jejuni/cytology; Campylobacter jejuni/genetics; Campylobacter jejuni/growth & development; Campylobacter jejuni/physiology; Carbon-Sulfur Lyases; Colony Count, Microbial; Humans; Luminescent Measurements; Molecular Sequence Data; Mutation; Oxidative Stress; Phenotype; Sequence Homology, Amino Acid; Signal Transduction |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0043768: S-ribosylhomocysteine lyase activity |
ECO:0000315: |
F |
IMP based on Fig. 2 and Figure 3 evidence; Figure 2 shows the production of an extracellular signalling molecule with AI-2-like activity by C. jejuni NCTC 11168; Figure 3 shows that Cj1198 can generate AI-2-like activity in a heterologous host when the addition of cell-free culture medium from cells containing pKE25 stimulated luminescence in a manner similar to the positive control (V. harveyi BB152). |
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