PMID:11929974

Citation	van Hemert, MJ, Lamers, GE, Klein, DC, Oosterkamp, TH, Steensma, HY and van Heusden, GP (2002) The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies. Proc. Natl. Acad. Sci. U.S.A. 99:5390-3
Abstract	The FIN1 gene from the yeast Saccharomyces cerevisiae encodes a basic protein with putative coiled-coil regions. Here we show that in large-budded cells a green fluorescent protein-Fin1 fusion protein is visible as a filament between the two spindle pole bodies. In resting cells the protein is undetectable, and in small-budded cells it is localized in the nucleus. During late mitosis it localizes on the spindle pole bodies. Filaments of cyano fluorescent protein-tagged Fin1 colocalize with filaments of green fluorescent protein-tagged Tub1 only in large-budded cells. By electron and atomic force microscopy we showed that purified recombinant Fin1p self-assembles into filaments with a diameter of approximately 10 nm. Our results indicate that the Fin1 protein forms a cell cycle-specific filament, additional to the microtubules, between the spindle pole bodies of dividing yeast cells.
Links	PubMed PMC122779 Online version:10.1073/pnas.072556099
Keywords	Cell Cycle; Cell Nucleus/metabolism; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/physiology; Green Fluorescent Proteins; Luminescent Proteins/metabolism; Microscopy, Atomic Force; Microscopy, Confocal; Microscopy, Electron; Microscopy, Fluorescence; Microtubules/metabolism; Mitosis; Mitotic Spindle Apparatus; Open Reading Frames; Protein Binding; Protein Structure, Tertiary; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/physiology; Two-Hybrid System Techniques
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