PMID:11921093

Citation	Vorísek, J, Techniková, Z, Schwippel, J and Benoist, P (2002) Enzymatic activities of Ura2 and Ura1 proteins (aspartate carbamoyltransferase and dihydro-orotate dehydrogenase) are present in both isolated membranes and cytoplasm of Saccharomyces cerevisiae. Yeast 19:449-57
Abstract	Computational analysis predicted three potential hydrophobic transmembrane alpha-helices within the Ura2 multidomain protein of Saccharomyces cerevisiae, the C-terminal subdomain of which catalyses the second step of uridine-monophosphate biosynthesis by its L-aspartate carbamoyltransferase activity (EC 2.1.3.2). The fourth step of pyrimidine biosynthesis is catalysed by dihydro-orotate dehydrogenase (Ura1 protein; EC 1.3.99.11), which was similarly characterized as a peripheral membrane protein. Ex situ, the activities of the investigated enzymes were associated both with isolated yeast membranes, fractionated by differential centrifugation to remove intact nuclei, and with soluble cytoplasmic proteins.
Links	PubMed
Keywords	Amino Acid Sequence; Aspartate Carbamoyltransferase/biosynthesis; Aspartate Carbamoyltransferase/metabolism; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/biosynthesis; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/metabolism; Cell Membrane/enzymology; Cytoplasm/enzymology; Fluorescent Dyes/chemistry; Indoles/chemistry; Molecular Sequence Data; Multienzyme Complexes/biosynthesis; Multienzyme Complexes/metabolism; Oxidoreductases/biosynthesis; Oxidoreductases/metabolism; Oxidoreductases Acting on CH-CH Group Donors; Protein Structure, Secondary; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.