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PMID:11914276
| Citation |
Kumar, A, Agarwal, S, Heyman, JA, Matson, S, Heidtman, M, Piccirillo, S, Umansky, L, Drawid, A, Jansen, R, Liu, Y, Cheung, KH, Miller, P, Gerstein, M, Roeder, GS and Snyder, M (2002) Subcellular localization of the yeast proteome. Genes Dev. 16:707-19 |
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| Abstract |
Protein localization data are a valuable information resource helpful in elucidating eukaryotic protein function. Here, we report the first proteome-scale analysis of protein localization within any eukaryote. Using directed topoisomerase I-mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of the Saccharomyces cerevisiae proteome. By high-throughput immunolocalization of tagged gene products, we have determined the subcellular localization of 2744 yeast proteins. Extrapolating these data through a computational algorithm employing Bayesian formalism, we define the yeast localizome (the subcellular distribution of all 6100 yeast proteins). We estimate the yeast proteome to encompass approximately 5100 soluble proteins and >1000 transmembrane proteins. Our results indicate that 47% of yeast proteins are cytoplasmic, 13% mitochondrial, 13% exocytic (including proteins of the endoplasmic reticulum and secretory vesicles), and 27% nuclear/nucleolar. A subset of nuclear proteins was further analyzed by immunolocalization using surface-spread preparations of meiotic chromosomes. Of these proteins, 38% were found associated with chromosomal DNA. As determined from phenotypic analyses of nuclear proteins, 34% are essential for spore viability--a percentage nearly twice as great as that observed for the proteome as a whole. In total, this study presents experimentally derived localization data for 955 proteins of previously unknown function: nearly half of all functionally uncharacterized proteins in yeast. To facilitate access to these data, we provide a searchable database featuring 2900 fluorescent micrographs at http://ygac.med.yale.edu. |
| Links |
PubMed PMC155358 Online version:10.1101/gad.970902 |
| Keywords |
Algorithms; Cell Nucleus/metabolism; Chromosomes/metabolism; Cytoplasm/metabolism; Databases as Topic; Epitopes; Genome, Fungal; Microscopy, Fluorescence; Mitochondria/metabolism; Models, Genetic; Mutagenesis; Phenotype; Saccharomyces cerevisiae/metabolism; Software |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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