PMID:11906696

Citation	Ibañez-Tallon, I, Miwa, JM, Wang, HL, Adams, NC, Crabtree, GW, Sine, SM and Heintz, N (2002) Novel modulation of neuronal nicotinic acetylcholine receptors by association with the endogenous prototoxin lynx1. Neuron 33:893-903
Abstract	We previously identified lynx1 as a neuronal membrane molecule related to snake alpha-neurotoxins able to modulate nAChRs. Here, we show that lynx1 colocalizes with nAChRs on CNS neurons and physically associates with nAChRs. Single-channel recordings show that lynx1 promotes the largest of three current amplitudes elicited by ACh through alpha(4)beta(2) nAChRs and that lynx1 enhances desensitization. Macroscopic recordings quantify the enhancement of desensitization onset by lynx1 and further show that it slows recovery from desensitization and increases the EC(50). These experiments establish that direct interaction of lynx1 with nAChRs can result in a novel type of functional modulation and suggest that prototoxins may play important roles in vivo by modulating functional properties of their cognate CNS receptors.
Links	PubMed
Keywords	Acetylcholine/pharmacology; Animals; Cell Line; GPI-Linked Proteins; Humans; Immunohistochemistry; Membrane Glycoproteins/chemistry; Membrane Glycoproteins/genetics; Membrane Glycoproteins/metabolism; Neurons/cytology; Neurons/drug effects; Neurons/metabolism; Neuropeptides/chemistry; Neuropeptides/genetics; Neuropeptides/metabolism; Oocytes/drug effects; Oocytes/physiology; Patch-Clamp Techniques; Protein Structure, Tertiary; Protein Subunits; Receptors, GABA-A/genetics; Receptors, GABA-A/metabolism; Receptors, Nicotinic/genetics; Receptors, Nicotinic/metabolism; Transfection; Vasodilator Agents; Xenopus laevis/physiology
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