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PMID:11861554
| Citation |
Rinaldo, C, Bazzicalupo, P, Ederle, S, Hilliard, M and La Volpe, A (2002) Roles for Caenorhabditis elegans rad-51 in meiosis and in resistance to ionizing radiation during development. Genetics 160:471-9 |
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| Abstract |
We have investigated the role of Caenorhabditis elegans RAD-51 during meiotic prophase and embryogenesis, making use of the silencing effect of RNA interference (RNAi). rad-51 RNAi leads to severe defects in chromosome morphology in diakinesis oocytes. We have explored the effect of rad-51 RNAi in mutants lacking fundamental components of the recombination machinery. If double-strand breaks are prevented by spo-11 mutation, rad-51 RNAi does not affect chromosome appearance. This is consistent with a role for RAD-51 downstream of the initiation of recombination. In the absence of MRE-11, as in the absence of SPO-11, RAD-51 depletion has no effect on the chromosomes, which appear intact, thus indicating a role for MRE-11 in DSB induction. Intriguingly, rad-51 silencing in oocytes that lack MSH-5 leads to chromosome fragmentation, a novel trait that is distinct from that seen in msh-5 mutants and in rad-51 RNAi oocytes, suggesting new potential roles for the msh-5 gene. Silencing of the rad-51 gene also causes a reduction in fecundity, which is suppressed by mutation in the DNA damage checkpoint gene rad-5, but not in the cell death effector gene ced-3. Finally, RAD-51 depletion is also seen to affect the soma, resulting in hypersensitivity to ionizing radiation in late embryogenesis. |
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| Keywords |
Animals; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/physiology; Caenorhabditis elegans/radiation effects; Caenorhabditis elegans Proteins; Chromosome Aberrations/embryology; Chromosome Aberrations/radiation effects; DNA-Binding Proteins/physiology; Embryo, Nonmammalian/embryology; Embryo, Nonmammalian/radiation effects; Gamma Rays; Meiosis/physiology; Meiosis/radiation effects; RNA, Messenger/metabolism; Rad51 Recombinase; Radiation Tolerance/genetics |
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