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PMID:11844802
| Citation |
Smardon, AM, Tarsio, M and Kane, PM (2002) The RAVE complex is essential for stable assembly of the yeast V-ATPase. J. Biol. Chem. 277:13831-9 |
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| Abstract |
Vacuolar proton-translocating ATPases are composed of a peripheral complex, V(1), attached to an integral membrane complex, V(o). Association of the two complexes is essential for ATP-driven proton transport and is regulated post-translationally in response to glucose concentration. A new complex, RAVE, was recently isolated and implicated in glucose-dependent reassembly of V-ATPase complexes that had disassembled in response to glucose deprivation (Seol, J. H., Shevchenko, A., and Deshaies, R. J. (2001) Nat. Cell Biol. 3, 384-391). Here, we provide evidence supporting a role for RAVE in reassembly of the V-ATPase but also demonstrate an essential role in V-ATPase assembly under other conditions. The RAVE complex associates reversibly with V(1) complexes released from the membrane by glucose deprivation but binds constitutively to cytosolic V(1) sectors in a mutant lacking V(o) sectors. V-ATPase complexes from cells lacking RAVE subunits show serious structural and functional defects even in glucose-grown cells or in combination with a mutation that blocks disassembly of the V-ATPase. RAVE small middle dotV(1) interactions are specifically disrupted in cells lacking V(1) subunits E or G, suggesting a direct involvement for these subunits in interaction of the two complexes. Skp1p, a RAVE subunit involved in many different signal transduction pathways, binds stably to other RAVE subunits under conditions that alter RAVE small middle dotV(1) binding; thus, Skp1p recruitment to the RAVE complex does not appear to provide a signal for V-ATPase assembly. |
| Links |
PubMed Online version:10.1074/jbc.M200682200 |
| Keywords |
Cell Cycle Proteins/metabolism; Cell Membrane/metabolism; Fungal Proteins/metabolism; Genotype; Glucose/metabolism; Models, Biological; Mutation; Precipitin Tests; Protein Binding; S-Phase Kinase-Associated Proteins; Signal Transduction; Vacuolar Proton-Translocating ATPases/chemistry; Vacuolar Proton-Translocating ATPases/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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