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Winston, WM, Molodowitch, C and Hunter, CP (2002) Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1. Science 295:2456-9


Double-stranded RNA-mediated gene interference (RNAi) in Caenorhabditis elegans systemically inhibits gene expression throughout the organism. To investigate how gene-specific silencing information is transmitted between cells, we constructed a strain that permits visualization of systemic RNAi. We used this strain to identify systemic RNA interference-deficient (sid) loci required to spread gene-silencing information between tissues but not to initiate or maintain an RNAi response. One of these loci, sid-1, encodes a conserved protein with predicted transmembrane domains. SID-1 is expressed in cells sensitive to RNAi, is localized to the cell periphery, and is required cell-autonomously for systemic RNAi.


PubMed Online version:10.1126/science.1068836


Amino Acid Sequence; Animals; Animals, Genetically Modified; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/chemistry; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/physiology; Calmodulin-Binding Proteins/genetics; Cytoplasm/metabolism; Embryo, Nonmammalian/physiology; Gene Silencing; Genes, Helminth; Germ Cells/metabolism; Green Fluorescent Proteins; Intestines/metabolism; Luminescent Proteins/genetics; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/physiology; Molecular Sequence Data; Mosaicism; Muscle Proteins/genetics; Muscles/metabolism; Mutation; Protein Structure, Tertiary; RNA, Double-Stranded/genetics; RNA, Double-Stranded/metabolism; RNA, Helminth/genetics; RNA, Helminth/metabolism; Recombinant Fusion Proteins/metabolism; Transgenes



Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

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