GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:11818540
| Citation |
Maita, N, Okada, K, Hatakeyama, K and Hakoshima, T (2002) Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP. Proc. Natl. Acad. Sci. U.S.A. 99:1212-7 |
|---|---|
| Abstract |
In the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin. The crystal structure of the stimulatory complex reveals that the GTPCHI decamer is sandwiched by two GFRP homopentamers. Each GFRP pentamer forms a symmetrical five-membered ring similar to beta-propeller. Five phenylalanine molecules are buried inside each interface between GFRP and GTPCHI, thus enhancing the binding of these proteins. The complex structure suggests that phenylalanine-induced GTPCHI x GFRP complex formation enhances GTPCHI activity by locking the enzyme in the active state. |
| Links |
PubMed PMC122169 Online version:10.1073/pnas.022646999 |
| Keywords |
Amino Acid Sequence; Animals; Crystallography, X-Ray; Enzyme Inhibitors/chemistry; Escherichia coli/genetics; GTP Cyclohydrolase/antagonists & inhibitors; GTP Cyclohydrolase/chemistry; Models, Molecular; Molecular Sequence Data; Protein Conformation; Protein Structure, Secondary; Proteins/chemistry; Rats; Recombinant Proteins/chemistry; Sequence Alignment; Sequence Homology, Amino Acid |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
