PMID:11756663

Citation	Torres, MA, Dangl, JL and Jones, JD (2002) Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response. Proc. Natl. Acad. Sci. U.S.A. 99:517-22
Abstract	Reactive oxygen intermediates (ROI) are strongly associated with plant defense responses. The origin of these ROI has been controversial. Arabidopsis respiratory burst oxidase homologues (rboh genes) have been proposed to play a role in ROI generation. We analyzed lines carrying dSpm insertions in the highly expressed AtrbohD and AtrbohF genes. Both are required for full ROI production observed during incompatible interactions with the bacterial pathogen Pseudomonas syringae pv. tomato DC3000(avrRpm1) and the oomycete parasite Peronospora parasitica. We also observed reduced cell death, visualized by trypan blue stain and reduced electrolyte leakage, in the Atrboh mutants after DC3000(avrRpm1) inoculation. However, enhanced cell death is observed after infection of mutant lines with P. parasitica. Paradoxically, although atrbohD mutation eliminated the majority of total ROI production, atrbohF mutation exhibited the strongest effect on cell death.
Links	PubMed PMC117592 Online version:10.1073/pnas.012452499
Keywords	Arabidopsis/genetics; Arabidopsis/microbiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/physiology; Cell Death; Hydrogen Peroxide/metabolism; Membrane Glycoproteins/genetics; Membrane Glycoproteins/physiology; Models, Genetic; Mutagenesis; Mutation; NADPH Oxidase; Nitric Oxide/metabolism; Plant Diseases/microbiology; Plant Physiological Phenomena; Reactive Oxygen Species; Time Factors
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.