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PMID:11697904

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Citation

Rydman, PS, Bamford, JK and Bamford, DH (2001) A minor capsid protein P30 is essential for bacteriophage PRD1 capsid assembly. J. Mol. Biol. 313:785-95

Abstract

Bacteriophage PRD1 is a double-stranded DNA virus infecting Gram-negative hosts. It has a membrane component located in the interior of the isometric capsid. In addition to the major capsid protein P3, the capsid contains a 9 kDa protein P30. Protein P30 is proposed to be located between the adjacent facets of the icosahedral capsid and is required for stable capsid assembly. In its absence, an empty phage-specific membrane vesicle is formed. The major protein component of this vesicle is a phage-encoded assembly factor, protein P10, that is not present in the final structure.

Links

PubMed Online version:10.1006/jmbi.2001.5068

Keywords

Bacteriophage PRD1/chemistry; Bacteriophage PRD1/genetics; Bacteriophage PRD1/metabolism; Bacteriophage PRD1/ultrastructure; Capsid/chemistry; Capsid/genetics; Capsid/metabolism; Capsid/ultrastructure; Centrifugation, Density Gradient; Escherichia coli/virology; Genetic Complementation Test; Microscopy, Electron; Mutation/genetics; Salmonella enterica/virology; Virion/chemistry; Virion/genetics; Virion/metabolism; Virion/ultrastructure; Virus Assembly

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPPRD:CAPSD

GO:0055036: virion membrane

ECO:0000314:

C

Figure 1.

complete
CACAO 6818

BPPRD:VP11

GO:0019012: virion

ECO:0000314:

C

Figure 3.

complete
CACAO 6829

BPPRD:VP11

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:P2

GO:0019012: virion

ECO:0000314:

C

Figure 1.

complete
CACAO 6285

BPPRD:P2

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:P5

GO:0019012: virion

ECO:0000314:

C

Figure 1.

complete
CACAO 6282

BPPRD:P5

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:VP30

GO:0019012: virion

ECO:0000314:

C

Figure 1.

complete
CACAO 6258

BPPRD:VP30

GO:0019069: viral capsid assembly

ECO:0000315:

P

Figure 2 & 3.

complete
CACAO 6824

BPPRD:VP30

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:VP30

involved_in

GO:0019069: viral capsid assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

BPPRD:VP31

GO:0019028: viral capsid

ECO:0000314:

C

Figure 1.

complete
CACAO 6841

BPPRD:VP31

part_of

GO:0019028: viral capsid

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:EXLYS

GO:0019012: virion

ECO:0000314:

C

Figure 3.

complete
CACAO 6825

BPPRD:EXLYS

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:PKG6

GO:0019012: virion

ECO:0000314:

C

Figure 1.

complete
CACAO 6280

BPPRD:PKG6

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:PKG9

GO:0019012: virion

ECO:0000314:

C

Figure 3.

complete
CACAO 6826

BPPRD:PKG9

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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