PMID:11591658

Citation	Zhang, Y, Pohlmann, EL, Ludden, PW and Roberts, GP (2001) Functional characterization of three GlnB homologs in the photosynthetic bacterium Rhodospirillum rubrum: roles in sensing ammonium and energy status. J. Bacteriol. 183:6159-68
Abstract	The GlnB (P(II)) protein, the product of glnB, has been characterized previously in the photosynthetic bacterium Rhodospirillum rubrum. Here we describe identification of two other P(II) homologs in this organism, GlnK and GlnJ. Although the sequences of these three homologs are very similar, the molecules have both distinct and overlapping functions in the cell. While GlnB is required for activation of NifA activity in R. rubrum, GlnK and GlnJ do not appear to be involved in this process. In contrast, either GlnB or GlnJ can serve as a critical element in regulation of the reversible ADP ribosylation of dinitrogenase reductase catalyzed by the dinitrogenase reductase ADP-ribosyl transferase (DRAT)/dinitrogenase reductase-activating glycohydrolase (DRAG) regulatory system. Similarly, either GlnB or GlnJ is necessary for normal growth on a variety of minimal and rich media, and any of the proteins is sufficient for normal posttranslational regulation of glutamine synthetase. Surprisingly, in their regulation of the DRAT/DRAG system, GlnB and GlnJ appeared to be responsive not only to changes in nitrogen status but also to changes in energy status, revealing a new role for this family of regulators in central metabolic regulation.
Links	PubMed PMC100091 Online version:10.1128/JB.183.21.6159-6168.2001
Keywords	ADP Ribose Transferases/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Carrier Proteins/genetics; Carrier Proteins/physiology; Cloning, Molecular; Darkness; Energy Metabolism; Glutamate-Ammonia Ligase/metabolism; Glycoside Hydrolases/metabolism; Models, Biological; Molecular Sequence Data; Mutation; N-Glycosyl Hydrolases; NAD/pharmacology; Nitrogen Fixation; Nitrogenase/metabolism; PII Nitrogen Regulatory Proteins; Quaternary Ammonium Compounds/pharmacology; Rhodospirillum rubrum/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RHORU:Q9L8F2	enables	GO:0008882: [glutamate-ammonia-ligase] adenylyltransferase activity	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:Q53044 UniProtKB:Q9AM71	F		Seeded From UniProt	complete
RHORU:Q9L8F2		GO:0008882: (glutamate-ammonia-ligase) adenylyltransferase activity	ECO:0000316:	UniProtKB:Q53044 UniProtKB:Q9AM71	F		See Figure 1, associated paragraph in results section.	complete CACAO 3396

References

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