PMID:11559569

Citation	Sohn, KJ, Shah, SA, Reid, S, Choi, M, Carrier, J, Comiskey, M, Terhorst, C and Kim, YI (2001) Molecular genetics of ulcerative colitis-associated colon cancer in the interleukin 2- and beta(2)-microglobulin-deficient mouse. Cancer Res. 61:6912-7
Abstract	Mice deficient in beta(2)-microglobulin and interleukin 2 (beta(2)m(null) x IL-2(null)) spontaneously develop colon cancer in the setting of chronic ulcerative colitis (UC). We investigated mutations of the Apc and p53 genes and microsatellite instability in colonic adenocarcinomas arising in this model. Mutations of the Apc and p53 genes in the regions corresponding to mutation hot spots in human colorectal cancer were determined by sequencing in 11 colonic adenocarcinomas. Microsatellite instability was determined in matched normal and neoplastic DNA at five loci. All 11 adenocarcinomas harbored Apc mutations. Of these 11 tumors, 5 harbored truncating mutations. A total of 67 Apc mutations were found in these 11 tumors; 59 were missense mutations, whereas 8 were frameshift or nonsense mutations. Six of the 11 adenocarcinomas harbored p53 mutations. A total of seven p53 mutations were found in these 11 tumors; all mutations were transitions, 4 of which were C:G-->T:A transitions occurring in codon 229 at cytosine-guanine dinucleotides. Nine adenocarcinomas exhibited microsatellite instability in at least one of the five loci examined; 1 tumor had microsatellite instability in two loci. Molecular genetics, as well as clinical features, of colon cancer in the beta(2)m(null) x IL-2(null) mice are similar to those of human UC-associated colorectal cancer. As such, this model appears to be an excellent animal model to study UC-associated colorectal carcinogenesis.
Links	PubMed
Keywords	Adenocarcinoma/complications; Adenocarcinoma/genetics; Animals; Colitis, Ulcerative/complications; Colitis, Ulcerative/genetics; Colonic Neoplasms/complications; Colonic Neoplasms/genetics; Crosses, Genetic; Disease Models, Animal; Frameshift Mutation; Genes, APC/genetics; Genes, p53/genetics; Interleukin-2/deficiency; Mice; Mice, Inbred C57BL; Microsatellite Repeats/genetics; Mutation, Missense; beta 2-Microglobulin/deficiency
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.