PMID:11546872

Citation	Puthalakath, H, Villunger, A, O'Reilly, LA, Beaumont, JG, Coultas, L, Cheney, RE, Huang, DC and Strasser, A (2001) Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science 293:1829-32
Abstract	Bcl-2 family members bearing only the BH3 domain are essential inducers of apoptosis. We identified a BH3-only protein, Bmf, and show that its BH3 domain is required both for binding to prosurvival Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf is sequestered to myosin V motors by association with dynein light chain 2. Certain damage signals, such as loss of cell attachment (anoikis), unleash Bmf, allowing it to translocate and bind prosurvival Bcl-2 proteins. Thus, at least two mammalian BH3-only proteins, Bmf and Bim, function to sense intracellular damage by their localization to distinct cytoskeletal structures.
Links	PubMed Online version:10.1126/science.1062257
Keywords	Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; Anoikis; Apoptosis Regulatory Proteins; Calmodulin-Binding Proteins/metabolism; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Line; Cytoskeleton/metabolism; Drosophila Proteins; Dyneins; Gene Expression Profiling; Humans; Membrane Proteins; Mice; Molecular Motor Proteins/metabolism; Molecular Sequence Data; Mutation; Myosin Type V; Neoplasm Proteins/genetics; Neoplasm Proteins/metabolism; Nerve Tissue Proteins/metabolism; Protein Binding; Protein Structure, Tertiary; Protein Transport; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-bcl-2/chemistry; Proto-Oncogene Proteins c-bcl-2/genetics; Proto-Oncogene Proteins c-bcl-2/metabolism; RNA, Messenger/analysis; RNA, Messenger/genetics; Transfection; Two-Hybrid System Techniques
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