GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:11514522

From GONUTS
Jump to: navigation, search
Citation

Sakamoto, K, Margolles, A, van Veen, HW and Konings, WN (2001) Hop resistance in the beer spoilage bacterium Lactobacillus brevis is mediated by the ATP-binding cassette multidrug transporter HorA. J. Bacteriol. 183:5371-5

Abstract

Lactobacillus brevis is a major contaminant of spoiled beer. The organism can grow in beer in spite of the presence of antibacterial hop compounds that give the beer a bitter taste. The hop resistance in L. brevis is, at least in part, dependent on the expression of the horA gene. The deduced amino acid sequence of HorA is 53% identical to that of LmrA, an ATP-binding cassette multidrug transporter in Lactococcus lactis. To study the role of HorA in hop resistance, HorA was functionally expressed in L. lactis as a hexa-histidine-tagged protein using the nisin-controlled gene expression system. HorA expression increased the resistance of L. lactis to hop compounds and cytotoxic drugs. Drug transport studies with L. lactis cells and membrane vesicles and with proteoliposomes containing purified HorA protein identified HorA as a new member of the ABC family of multidrug transporters.

Links

PubMed PMC95421

Keywords

ATP-Binding Cassette Transporters/genetics; ATP-Binding Cassette Transporters/metabolism; Acids/metabolism; Acids/pharmacology; Beer/microbiology; Benzimidazoles/metabolism; Drug Resistance, Microbial/genetics; Drug Resistance, Multiple/genetics; Ethidium/metabolism; Food Microbiology; Genes, Bacterial; Lactobacillus/drug effects; Lactobacillus/genetics; Lactobacillus/metabolism; Lactococcus lactis/genetics; Lactococcus lactis/metabolism; Liposomes/metabolism; Rosales/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

LACBR:O32748

GO:0042908: xenobiotic transport

ECO:0000315:

P

The de-energization by Ortho-vandate led to significant decrease in ethidium efflux (Figure 3).

complete

LACBR:O32748

GO:0071236: cellular response to antibiotic

ECO:0000250:

UniProtKB:Q9CHL8


P

HorA shows 53% homology with the ATP-binding cassette multidrug transporter LmrA

complete


See also

References

See Help:References for how to manage references in GONUTS.