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Beck, K, Eisner, G, Trescher, D, Dalbey, RE, Brunner, J and Müller, M (2001) YidC, an assembly site for polytopic Escherichia coli membrane proteins located in immediate proximity to the SecYE translocon and lipids. EMBO Rep. 2:709-14


Like its mitochondrial homolog Oxa1p, the inner membrane protein YidC of Escherichia coli is involved in the integration of membrane proteins. We have analyzed individual insertion steps of the polytopic E. coli membrane protein MtlA targeted as ribosome-nascent chain complexes to inner membrane vesicles. YidC can accommodate at least the first two transmembrane segments of MtlA at the protein lipid interface and retain them even though the length of the nascent chain would amply allow insertion into membrane lipids. An even longer insertion intermediate of MtlA is described that still has the first transmembrane helix bound to YidC while the third contacts SecE and YidC during integration. Our findings suggest that YidC forms a contiguous integration unit with the SecYE translocon and functions as an assembly site for polytopic membrane proteins mediating the formation of helix bundles prior to their release into the membrane lipids.


PubMed PMC1083991 Online version:10.1093/embo-reports/kve154


Bacterial Proteins/metabolism; Escherichia coli/physiology; Escherichia coli Proteins; Membrane Lipids/metabolism; Membrane Proteins/biosynthesis; Membrane Proteins/chemistry; Membrane Transport Proteins; Monosaccharide Transport Proteins; Phosphoenolpyruvate Sugar Phosphotransferase System/biosynthesis; Phosphoenolpyruvate Sugar Phosphotransferase System/chemistry; Protein Biosynthesis



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status


GO:0032977: membrane insertase activity



figures 1, 2, and 3


See also


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