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PMID:11461699
| Citation |
Grishok, A, Pasquinelli, AE, Conte, D, Li, N, Parrish, S, Ha, I, Baillie, DL, Fire, A, Ruvkun, G and Mello, CC (2001) Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell 106:23-34 |
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| Abstract |
RNAi is a gene-silencing phenomenon triggered by double-stranded (ds) RNA and involves the generation of 21 to 26 nt RNA segments that guide mRNA destruction. In Caenorhabditis elegans, lin-4 and let-7 encode small temporal RNAs (stRNAs) of 22 nt that regulate stage-specific development. Here we show that inactivation of genes related to RNAi pathway genes, a homolog of Drosophila Dicer (dcr-1), and two homologs of rde-1 (alg-1 and alg-2), cause heterochronic phenotypes similar to lin-4 and let-7 mutations. Further we show that dcr-1, alg-1, and alg-2 are necessary for the maturation and activity of the lin-4 and let-7 stRNAs. Our findings suggest that a common processing machinery generates guide RNAs that mediate both RNAi and endogenous gene regulation. |
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| Keywords |
Animals; Animals, Genetically Modified; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/growth & development; DNA Primers; Drosophila/genetics; Embryo, Nonmammalian/physiology; Female; Gene Expression Regulation, Developmental; Gene Silencing; Genes, Helminth; Genes, Reporter; Genomic Imprinting; Heterozygote; Larva; Luciferases/genetics; Phylogeny; Polymerase Chain Reaction; RNA, Helminth/genetics |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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