PMID:11401975

Citation	Shankar, N, Lockatell, CV, Baghdayan, AS, Drachenberg, C, Gilmore, MS and Johnson, DE (2001) Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection. Infect. Immun. 69:4366-72
Abstract	Enterococcus faecalis bacteria isolated from patients with bacteremia, endocarditis, and urinary tract infections more frequently express the surface protein Esp than do fecal isolates. To assess the role of Esp in colonization and persistence of E. faecalis in an animal model of ascending urinary tract infection, we compared an Esp(+) strain of E. faecalis to its isogenic Esp-deficient mutant. Groups of CBA/J mice were challenged transurethrally with 10(8) CFU of either the parent or mutant strain, and bacteria in the urine, bladder, and kidneys were enumerated 5 days postinfection. Significantly higher numbers of bacteria were recovered from the bladder and urine of mice challenged with the parent strain than from the bladder and urine of mice challenged with the mutant. Colonization of the kidney, however, was not significantly different between the parent and mutant strains. Histopathological evaluations of kidney and bladder tissue done at 5 days postinfection did not show marked histopathological changes consistent with inflammation, mucosal hyperplasia, or apoptosis, and there was no observable difference between the mice challenged with the parent and those challenged with the mutant. We conclude that, while Esp does not influence histopathological changes associated with acute urinary tract infections, it contributes to colonization and persistence of E. faecalis at this site.
Links	PubMed PMC98508 Online version:10.1128/IAI.69.7.4366-4372.2001
Keywords	Animals; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Disease Models, Animal; Enterococcus faecalis/genetics; Enterococcus faecalis/pathogenicity; Gram-Positive Bacterial Infections/microbiology; Humans; Membrane Proteins/genetics; Membrane Proteins/physiology; Mice; Mice, Inbred CBA; Phenotype; Rabbits; Urinary Tract Infections/microbiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ENTFL:Q0MUV3		GO:0009405: pathogenesis	ECO:0000315:		P		Figure 5. shows decreased CFU counts in the urine, bladder, and kidneys of mice challenged with Esp-deficient E.faecalis.	complete CACAO 5999
ENTFL:Q0MUV3	involved_in	GO:0009405: pathogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

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