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PMID:11389436
| Citation |
Antonny, B, Madden, D, Hamamoto, S, Orci, L and Schekman, R (2001) Dynamics of the COPII coat with GTP and stable analogues. Nat. Cell Biol. 3:531-7 |
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| Abstract |
We have developed an assay to monitor the assembly of the COPII coat onto liposomes in real time. We show that with Sar1pGTP bound to liposomes, a single round of assembly and disassembly of the COPII coat lasts a few seconds. The two large COPII complexes Sec23/24p and Sec13/31p bind almost instantaneously (in less than 1 s) to Sar1pGTP-doped liposomes. This binding is followed by a fast (less than 10 s) disassembly due to a 10-fold acceleration of the GTPase-activating protein activity of Sec23/24p by the Sec13/31p complex. Experiments with the phosphate analogue BeFx suggest that Sec23/24p provides residues directly involved in GTP hydrolysis on Sar1p. |
| Links |
PubMed Online version:10.1038/35078500 |
| Keywords |
COP-Coated Vesicles/metabolism; Fungal Proteins/metabolism; GTPase-Activating Proteins; Guanosine Triphosphate/metabolism; Liposomes/metabolism; Monomeric GTP-Binding Proteins/metabolism; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; Temperature; Vesicular Transport Proteins |
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Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
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