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PMID:11358866
| Citation |
Moriya, H, Shimizu-Yoshida, Y, Omori, A, Iwashita, S, Katoh, M and Sakai, A (2001) Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal. Genes Dev. 15:1217-28 |
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| Abstract |
POP2 protein of Saccharomyces cerevisiae is a component of a protein complex that regulates the transcription of many genes. We found that the 97th threonine residue (Thr 97) of Pop2p was phosphorylated upon glucose limitation. The Thr 97 phosphorylation occurred within 2 min after removing glucose and was reversed within 1 min after the readdition of glucose. The effects of hexokinase mutations and glucose analogs indicate that this phosphorylation is dependent on glucose phosphorylating activity. We purified a protein kinase that phosphorylates a peptide containing Thr 97 of Pop2p and identified it as Yak1p, a DYRK family kinase. Phosphorylation of Pop2p was barely detectable in a yak1Delta strain. We found that Yak1p interacted with Bmh1p and Bmh2p only in the presence of glucose. A GFP-Yak1p fusion protein shuttled rapidly between the nucleus and the cytoplasm in response to glucose. A strain with alanine substituted for Thr 97 in Pop2p showed overgrowth in the postdiauxic transition and failed to stop the cell cycle at G(1) phase in response to glucose deprivation. Thus, Yak1p and Pop2p are part of a novel glucose-sensing system in yeast that is involved in growth control in response to glucose availability. |
| Links |
PubMed PMC313799 Online version:10.1101/gad.884001 |
| Keywords |
Carrier Proteins/metabolism; Cell Division; Cell Nucleus/metabolism; Electrophoresis, Polyacrylamide Gel; Glucose/metabolism; Intracellular Signaling Peptides and Proteins; Phosphorylation; Protein-Serine-Threonine Kinases/isolation & purification; Protein-Serine-Threonine Kinases/metabolism; Protein-Tyrosine Kinases/isolation & purification; Protein-Tyrosine Kinases/metabolism; Ribonucleases; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; Signal Transduction; Transcription Factors/metabolism |
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Significance
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| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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