PMID:11330860

Citation	Azhar, M, Kennady, PK, Pande, G, Espiritu, M, Holloman, W, Brazill, D, Gomer, RH and Nanjundiah, V (2001) Cell cycle phase, cellular Ca2+ and development in Dictyostelium discoideum. Int. J. Dev. Biol. 45:405-14
Abstract	In Dictyostelium discoideum, the initial differentiation of cells is regulated by the phase of the cell cycle at starvation. Cells in S and early G2 (or with a low DNA content) have relatively high levels of cellular Ca2+ and display a prestalk tendency after starvation, whereas cells in mid to late G2 (or with a high DNA content) have relatively low levels of Ca2+ and display a prespore tendency. We found that there is a correlation between cytosolic Ca2+ and cell cycle phase, with high Ca2+ levels being restricted to cells in the S and early G2 phases. As expected on the basis of this correlation, cell cycle inhibitors influence the proportions of amoebae containing high or low Ca2+. However, it has been reported that in the rtoA mutant, which upon differentiation gives rise to many more stalk cells than spores (compared to the wild type), initial cell-type choice is independent of cell cycle phase at starvation. In contrast to the wild type, a disproportionately large fraction of rtoA amoebae fall into the high Ca2+ class, possibly due to an altered ability of this mutant to transport Ca2+.
Links	PubMed
Keywords	Animals; Calcium/metabolism; Cell Aggregation; Cell Cycle Proteins/metabolism; Cell Differentiation/physiology; DNA, Protozoan/analysis; Dictyostelium/cytology; Dictyostelium/physiology; Flow Cytometry; G2 Phase/physiology; Protozoan Proteins; S Phase/physiology; Spores
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