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PMID:11292810

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Citation

Cucarella, C, Solano, C, Valle, J, Amorena, B, Lasa, I and Penadés, JR (2001) Bap, a Staphylococcus aureus surface protein involved in biofilm formation. J. Bacteriol. 183:2888-96

Abstract

Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilm-producing Staphylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion mutants. Two mutants were found with a significant decrease in attachment to inert surfaces (early adherence), intercellular adhesion, and biofilm formation. The transposon was inserted at the same locus in both mutants. This locus (bap [for biofilm associated protein]) encodes a novel cell wall associated protein of 2,276 amino acids (Bap), which shows global organizational similarities to surface proteins of gram-negative (Pseudomonas aeruginosa and Salmonella enterica serovar Typhi) and gram-positive (Enteroccocus faecalis) microorganisms. Bap's core region represents 52% of the protein and consists of 13 successive nearly identical repeats, each containing 86 amino acids. bap was present in a small fraction of bovine mastitis isolates (5% of the 350 S. aureus isolates tested), but it was absent from the 75 clinical human S. aureus isolates analyzed. All staphylococcal isolates harboring bap were highly adherent and strong biofilm producers. In a mouse infection model bap was involved in pathogenesis, causing a persistent infection.

Links

PubMed PMC99507 Online version:10.1128/JB.183.9.2888-2896.2001

Keywords

Adhesins, Bacterial/chemistry; Adhesins, Bacterial/genetics; Adhesins, Bacterial/metabolism; Amino Acid Sequence; Amino Acids; Animals; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biofilms/growth & development; Cattle; Disease Models, Animal; Female; Humans; Mastitis, Bovine/microbiology; Mice; Molecular Sequence Data; Mutagenesis, Insertional; Staphylococcal Infections/microbiology; Staphylococcal Infections/veterinary; Staphylococcus aureus/chemistry; Staphylococcus aureus/genetics; Staphylococcus aureus/pathogenicity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

STAAU:Q79LN3

GO:0036164: cell-abiotic substrate adhesion

ECO:0000315:

P

Fig. 4 demonstrates that Bap impaired Staphylococcus aureus strains are unable to adhere to abiotic surfaces.

complete
CACAO 13306

STAAU:Q79LN3

GO:0098609: cell-cell adhesion

ECO:0000315:

P

Figure 4 “These results strongly suggest that Bap is not only involved in intercellular adhesion and accumulation in multilayered cell clusters, as the product of the ica operon does, but also in primary attachment to an abiotic surface.” Organism is Staphylococcus aureus. Uniprot:Bap, Biofilm-associated surface protein

complete
CACAO 13307

STAAU:Q79LN3

GO:0036164: cell-abiotic substrate adhesion

ECO:0000314:

P

Fig. 4 demonstrates that Bap impaired Staphylococcus aureus strains are unable to adhere to abiotic surfaces.

complete

STAAU:Q79LN3

GO:0090609: single-species submerged biofilm formation

ECO:0000315:

P

Figure 3 Unitprot: bap, biofilm association protein “Analysis of the capacity to form a biofilm (on a polystyrene surface after 24 h) demonstrated that all strains expressing Bap, including S. aureus SA113, a strain whose biofilm forma- tion has been related to the ica product (PIA-PNSG), showed an enhanced capacity to form a biofilm, implicating Bap in this process (Fig. 3).”

complete
CACAO 13308


See also

References

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