PMID:11252167

Citation	Isono, K, Abe, K, Tomaru, Y, Okazaki, Y, Hayashizaki, Y and Koseki, H (2001) Molecular cloning, genetic mapping, and expression of the mouse Sf3b1 (SAP155) gene for the U2 snRNP component of spliceosome. Mamm. Genome 12:192-8
Abstract	SAP155, a subunit of the U2 snRNP, is essential for prespliceosome assembly and splicing catalysis of the major spliceosome. Moreover, the protein has been identified in the minor (U12-dependent) spliceosome. These facts strongly suggest that SAP155 is shared by two distinct complexes owing to its importance in the removal of any type of intron. Here we have isolated a cDNA encoding the 146-kDa mouse homolog, designated Sf3b1. The amino acid sequence of Sf3b1 is very highly conserved among homologs from Schizosaccharomyces pombe (52.4% identity) to human (99.6%), and the C-terminal 825 residues of these Sf3b1 homologs show even higher identities. This C-terminal region shows significant similarity to the PR65 subunit of protein phosphatase 2A, which is composed of 15 tandem repeats of a 39 amino acid sequence. Mouse genome analyses showed Sf3bh1 to be a single-copy gene mapping to the central part of Chromosome (Chr) 1. Northern blot analysis and whole mount in situ hybridization revealed Sf3b1 to be ubiquitously expressed in a variety of adult tissues and mid-gestation embryos.
Links	PubMed Online version:10.1007/s003350010258
Keywords	Amino Acid Sequence; Animals; Base Sequence; Chromosome Mapping; Cloning, Molecular; Conserved Sequence; DNA, Complementary; Gene Dosage; Gene Expression; Humans; Mice; Molecular Sequence Data; Phosphoproteins/genetics; Ribonucleoprotein, U2 Small Nuclear/genetics; Sequence Homology, Amino Acid; Spliceosomes/metabolism
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.