PMID:11226170

Citation	Perrod, S, Cockell, MM, Laroche, T, Renauld, H, Ducrest, AL, Bonnard, C and Gasser, SM (2001) A cytosolic NAD-dependent deacetylase, Hst2p, can modulate nucleolar and telomeric silencing in yeast. EMBO J. 20:197-209
Abstract	In budding yeast, the silent information regulator Sir2p is a nuclear NAD-dependent deacetylase that is essential for both telomeric and rDNA silencing. All eukaryotic species examined to date have multiple homologues of Sir two (HSTs), which share a highly conserved globular core domain. Here we report that yeast Hst2p and a mammalian Hst2p homologue, hSirT2p, are cytoplasmic in yeast and human cells, in contrast to yHst1p and ySir2p which are exclusively nuclear. Although yHst2p cannot restore silencing in a sir2 deletion, overexpression of yHst2p influences nuclear silencing events in a SIR2 strain, derepressing subtelomeric silencing while increasing repression in the rDNA. In contrast, a form of ySir2p carrying a point mutation in the conserved core domain disrupts both telomeric position effect (TPE) and rDNA repression at low expression levels. This argues that non-nuclear yHst2p can compete for a substrate or ligand specifically required for telomeric, and not rDNA repression.
Links	PubMed PMC140183 Online version:10.1093/emboj/20.1.197
Keywords	Amidohydrolases/chemistry; Amidohydrolases/genetics; Amidohydrolases/metabolism; Amino Acid Substitution; Cell Nucleus/genetics; Cytosol/enzymology; DNA, Fungal/genetics; DNA, Ribosomal/genetics; Gene Silencing; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Phylogeny; Recombinant Proteins/metabolism; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sirtuin 2; Sirtuins; Telomere/genetics
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.