PMID:11214968

Citation	Kaneko, T, Nakamura, Y, Sato, S, Asamizu, E, Kato, T, Sasamoto, S, Watanabe, A, Idesawa, K, Ishikawa, A, Kawashima, K, Kimura, T, Kishida, Y, Kiyokawa, C, Kohara, M, Matsumoto, M, Matsuno, A, Mochizuki, Y, Nakayama, S, Nakazaki, N, Shimpo, S, Sugimoto, M, Takeuchi, C, Yamada, M and Tabata, S (2000) Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti. DNA Res. 7:331-8
Abstract	The complete nucleotide sequence of the genome of a symbiotic bacterium Mesorhizobium loti strain MAFF303099 was determined. The genome of M. loti consisted of a single chromosome (7,036,071 bp) and two plasmids, designated as pMLa (351,911 bp) and pMLb (208, 315 bp). The chromosome comprises 6752 potential protein-coding genes, two sets of rRNA genes and 50 tRNA genes representing 47 tRNA species. Fifty-four percent of the potential protein genes showed sequence similarity to genes of known function, 21% to hypothetical genes, and the remaining 25% had no apparent similarity to reported genes. A 611-kb DNA segment, a highly probable candidate of a symbiotic island, was identified, and 30 genes for nitrogen fixation and 24 genes for nodulation were assigned in this region. Codon usage analysis suggested that the symbiotic island as well as the plasmids originated and were transmitted from other genetic systems. The genomes of two plasmids, pMLa and pMLb, contained 320 and 209 potential protein-coding genes, respectively, for a variety of biological functions. These include genes for the ABC-transporter system, phosphate assimilation, two-component system, DNA replication and conjugation, but only one gene for nodulation was identified.
Links	PubMed
Keywords	ATP-Binding Cassette Transporters/genetics; Chromosomes/genetics; Codon; Conjugation, Genetic/genetics; DNA Replication/genetics; Genome, Bacterial; Molecular Sequence Data; Multigene Family; Nitrogen/metabolism; Phosphates/metabolism; Plasmids/genetics; Proteobacteria/genetics; RNA, Ribosomal/genetics; RNA, Transfer/genetics; Sequence Analysis, DNA
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RHILO:Y4317		GO:0005886: plasma membrane	ECO:0000250:	PMID:11214968	C		Matched 61.7% with Magnesium transport protein (Improved structure of Thermotoga maritima CorA at 2.7 A resolution). Figure 1 in the PubMed article shows the features of each assigned protein coding genes and their functional classifications. Plasmids of each gene in the chromosome are classified based on their similarities with genes of different functions. Genes were divided by sym and non sym based on where they lay in the chromosome. The different known function genes that were accounted for in the figure included amino acid biosynthesis, biosynthesis of cofactors, prosthetic groups, and carriers, cell envelope, cellular processes, central intermediary metabolism, cnergy metabolism, fatty acid, phospholipid and sterol metabolism, purines, pyrimidines, nucleosides, and nucleotides, regulatory functions, DNA replication, recombination, and repair, transcription, translation, transport and binding proteins, in addition to other categories.	complete CACAO 11879

References

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