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PMID:11207582
| Citation |
Guidi-Rontani, C, Weber-Levy, M, Mock, M and Cabiaux, V (2000) Translocation of Bacillus anthracis lethal and oedema factors across endosome membranes. Cell. Microbiol. 2:259-64 |
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| Abstract |
The two exotoxins of Bacillus anthracis, the causative agent of anthrax, are the oedema toxin (PA-EF) and the lethal toxin (PA-LF). They exert their catalytic activities within the cytosol. The internalization process requires receptor-mediated endocytosis and passage through acidic vesicles. We investigated the translocation of EF and LF enzymatic moieties across the target cell membrane. By selective permeabilization of the plasma membrane with Clostridium perfringens delta-toxin, we observed free full-size lethal factor (LF) within the cytosol, resulting from specific translocation from early endosomes. In contrast, oedema factor (EF) remained associated with the membranes of vesicles. |
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| Keywords |
Animals; Antigens, Bacterial; Bacillus anthracis/metabolism; Bacterial Toxins/metabolism; Carrier Proteins/physiology; Cell Line; Cell Membrane/physiology; Cytosol/metabolism; Endosomes/physiology; Macrophages/microbiology; Mice |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0044743: intracellular protein transmembrane import |
ECO:0000314: |
P |
See section 'EF remained associated with endosomal membrane after translocation', Figure 2(B), and Figure 4. Unlike LF, EF crosses the cell membrane enough to expose its active sites to the interior of the cell, but does not fully escape into the interior. |
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Notes
See also
References
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