PMID:11203704

Citation	Thatcher, JD, Fernandez, AP, Beaster-Jones, L, Haun, C and Okkema, PG (2001) The Caenorhabditis elegans peb-1 gene encodes a novel DNA-binding protein involved in morphogenesis of the pharynx, vulva, and hindgut. Dev. Biol. 229:480-93
Abstract	Gene expression in the Caenorhabditis elegans pharynx is regulated in part by organ-specific signals, which in the myo-2 gene target a regulatory sequence called the C sub-element. C sub-element activity requires the organ specification factor PHA-4, a winged-helix transcription factor expressed in all pharyngeal cells. To identify additional factors involved in pharyngeal organogenesis, we performed a yeast one-hybrid screen for C sub-element binding proteins. Here we describe the novel factor PEB-1, which is coexpressed with PHA-4 in many pharyngeal cell types, including muscles, epithelial cells, marginal cells, and glands, but is undetectable in the pharyngeal nervous system. PEB-1 is also detected outside the pharynx in cells surrounding the rectum and vulva, as well as in the germ line. Reduction of peb-1 function using RNAi results in morphological defects in the somatic tissues in which peb-1 is expressed. We have mapped the PEB-1 DNA-binding domain to a 158-residue region, which is unrelated to known DNA-binding proteins but shares some sequence similarity to the Drosophila Mod(mdg4) proteins. PEB-1 specifically recognizes a site in the C subelement that partially overlaps the PHA-4 binding site. Both the PEB-1 and the PHA-4 binding sites are necessary for strong C sub-element enhancer activity in some cells in which these factors are coexpressed. In contrast the PEB-1 site is dispensable for C sub-element activity in pharyngeal neurons. We propose that PEB-1 functions with PHA-4 to activate target gene expression in cells in which they are coexpressed.
Links	PubMed Online version:10.1006/dbio.2000.9978
Keywords	Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans Proteins; Cloning, Molecular; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Digestive System/embryology; Embryo, Nonmammalian/physiology; Female; Gene Library; Molecular Sequence Data; Morphogenesis; Pharynx/embryology; Promoter Regions, Genetic; Protein Isoforms/genetics; Protein Isoforms/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Vulva/embryology
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