PMID:11136982

Citation	Entchev, EV, Schwabedissen, A and González-Gaitán, M (2000) Gradient formation of the TGF-beta homolog Dpp. Cell 103:981-91
Abstract	Secreted morphogens such as the Drosophila TGF-beta homolog Decapentaplegic (Dpp) are thought to spread through target tissues and form long-range concentration gradients providing positional information. Using a GFP-Dpp fusion, we monitored a TGF-beta family member trafficking in situ throughout the target tissue and forming a long-range concentration gradient. Evidence is presented that long-range Dpp movement involves Dpp receptor and Dynamin functions. We also show that the rates of endocytic trafficking and degradation determine Dpp signaling range. We propose a model where the gradient is formed via intracellular trafficking initiated by receptor-mediated endocytosis of the ligand in receiving cells with the gradient slope controlled by endocytic sorting of Dpp toward recycling versus degradation.
Links	PubMed
Keywords	Animals; Animals, Genetically Modified; Body Patterning; Drosophila Proteins; Drosophila melanogaster/anatomy & histology; Drosophila melanogaster/embryology; Drosophila melanogaster/physiology; Dynamins; Endocytosis; Endosomes/metabolism; GTP Phosphohydrolases/genetics; GTP Phosphohydrolases/metabolism; Genes, Reporter; Green Fluorescent Proteins; Immunohistochemistry; Insect Proteins/genetics; Insect Proteins/metabolism; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Protein Transport/physiology; Recombinant Fusion Proteins/metabolism; Temperature; Transforming Growth Factor beta/chemistry; Transforming Growth Factor beta/metabolism; Wing/anatomy & histology; Wing/physiology; rab GTP-Binding Proteins/genetics; rab GTP-Binding Proteins/metabolism
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