PMID:11093124

Citation	Blanchard, AD, Page, KR, Watkin, H, Hayward, P, Wong, T, Bartholomew, M, Quint, DJ, Daly, M, Garcia-Lopez, J and Champion, BR (2000) Identification and characterization of SKAT-2, a novel Th2-specific zinc finger gene. Eur. J. Immunol. 30:3100-10
Abstract	We have identified a novel Kruppel-type zinc finger (ZF) gene, SKAT-2, which is selectively expressed by murine Th2 cells. The protein encoded by this gene has 14 C2H2-type ZF tandemly arrayed at its C terminus and N-terminal SCAN box and KRAB domains. SKAT-2 is tissue restricted in expression at the RNA level, detectable only in brain and at low levels in kidney and spleen and few hematopoietic cell lines. By in situ hybridization, SKAT-2 expression was found to peak in antigen-stimulated CD4(+) T cells after 2-3 days of culture under Th2 but not Th1 biasing conditions. This pattern of expression closely mirrored that of GATA-3 in the same cells. In transient transfection experiments in phorbol 12-myristate 13-acetate/ionomycin-stimulated EL4 cells, SKAT-2 was found to up-regulate the activity of the IL-4 but not the IL-5 promoter, contrasting with the ability of GATA-3 to activate both promoters. This result was confirmed using clones of EL4 cells stably expressing an inducible form of SKAT-2, thus SKAT-2 is a novel Th2-specific gene that may play a role in selective regulation of cytokine genes in T cells.
Links	PubMed Online version:10.1002/1521-4141(200011)30:11<3100::AID-IMMU3100>3.0.CO;2-W
Keywords	Amino Acid Sequence; Base Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/immunology; GATA3 Transcription Factor; Gene Expression/immunology; Humans; Molecular Sequence Data; Nerve Tissue Proteins; Proteins/genetics; Proteins/immunology; Th2 Cells/immunology; Trans-Activators/genetics; Trans-Activators/immunology; Zinc Fingers
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