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PMID:11083832
| Citation |
Borezee, E, Pellegrini, E and Berche, P (2000) OppA of Listeria monocytogenes, an oligopeptide-binding protein required for bacterial growth at low temperature and involved in intracellular survival. Infect. Immun. 68:7069-77 |
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| Abstract |
We identified a new oligopeptide permease operon in the pathogen Listeria monocytogenes. This opp operon consists of five genes (oppA, oppB, oppC, oppD, and oppF) and displays the same genetic organization as those of several bacterial species. The first gene of this operon, oppA, encodes a 62-kDa protein sharing 33% identity with OppA of Bacillus subtilis and is expressed predominantly during exponential growth. The function of oppA was studied by constructing an oppA deletion mutant. The phenotype analysis of this mutant revealed that OppA mediates the transport of oligopeptides and is required for bacterial growth at low temperature. The wild-type phenotype was restored by complementing the mutant with oppA. We also found that OppA is involved in intracellular survival in macrophages and in bacterial growth in organs of mice infected with L. monocytogenes, although the level of virulence was not altered in the mutant. These results show the major role of OppA in the uptake of oligopeptides and the pleiotropic effects of this oligopeptide-binding protein on the behavior of this pathogen in the environment and in its host. |
| Links | |
| Keywords |
Animals; Bacterial Proteins/physiology; Base Sequence; Carrier Proteins/physiology; Cloning, Molecular; Female; Lipoproteins/physiology; Listeria monocytogenes/growth & development; Macrophages/microbiology; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Oligopeptides/metabolism; Operon; Temperature; Transcription, Genetic |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016049: cell growth |
ECO:0000315: |
P |
Figure 5A shows growth at 5°C and oppA mutant. Figure 5B shows bacteria were grown to mid-log exponential phase at 37°C and transferred to 5°C until the end of growth. The growth was at 5°C and was restored in an oppA-complemented mutant. |
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Notes
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References
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