GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:11076749
| Citation |
Clifford, R, Lee, MH, Nayak, S, Ohmachi, M, Giorgini, F and Schedl, T (2000) FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the C. elegans hermaphrodite germline. Development 127:5265-76 |
|---|---|
| Abstract |
Male sex determination in the Caenorhabditis elegans hermaphrodite germline requires translational repression of tra-2 mRNA by the GLD-1 RNA binding protein. We cloned fog-2 by finding that its gene product physically interacts with GLD-1, forming a FOG-2/GLD-1/tra-2 3'untranslated region ternary complex. FOG-2 has an N-terminal F-box and a novel C-terminal domain called FTH. Canonical F-box proteins act as bridging components of the SCF ubiquitin ligase complex; the N-terminal F-box binds a Skp1 homolog, recruiting ubiquination machinery, while a C-terminal protein-protein interaction domain binds a specific substrate for degradation. However, since both fog-2 and gld-1 are necessary for spermatogenesis, FOG-2 cannot target GLD-1 for ubiquitin-mediated degradation. We propose that FOG-2 also acts as a bridge, bringing GLD-1 bound to tra-2 mRNA into a multiprotein translational repression complex, thus representing a novel function for an F-box protein. fog-2 is a member of a large, apparently rapidly evolving, C. elegans gene family that has expanded, in part, by local duplications; fog-2 related genes have not been found outside nematodes. fog-2 may have arisen during evolution of self-fertile hermaphroditism from an ancestral female/male species. |
| Links | |
| Keywords |
3' Untranslated Regions; Alleles; Amino Acid Sequence; Animals; Caenorhabditis elegans Proteins; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Disorders of Sex Development/genetics; Drosophila Proteins; Evolution, Molecular; Female; Genes, Helminth; Helminth Proteins/metabolism; Male; Molecular Sequence Data; Multigene Family; Protein Biosynthesis; RNA, Messenger/genetics; RNA-Binding Proteins/metabolism; Ribonucleoproteins/genetics; Sequence Homology, Amino Acid; Sex Determination Processes; Transcription Factors; Two-Hybrid System Techniques |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
