GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:11062264
| Citation |
Machado, C and Andrew, DJ (2000) D-Titin: a giant protein with dual roles in chromosomes and muscles. J. Cell Biol. 151:639-52 |
|---|---|
| Abstract |
Previously, we reported that chromosomes contain a giant filamentous protein, which we identified as titin, a component of muscle sarcomeres. Here, we report the sequence of the entire titin gene in Drosophila melanogaster, D-Titin, and show that it encodes a two-megadalton protein with significant colinear homology to the NH(2)-terminal half of vertebrate titin. Mutations in D-Titin cause chromosome undercondensation, chromosome breakage, loss of diploidy, and premature sister chromatid separation. Additionally, D-Titin mutants have defects in myoblast fusion and muscle organization. The phenotypes of the D-Titin mutants suggest parallel roles for titin in both muscle and chromosome structure and elasticity, and provide new insight into chromosome structure. |
| Links | |
| Keywords |
Animals; Cell Fusion; Chromatids/chemistry; Chromatids/genetics; Chromatids/metabolism; Chromosomal Proteins, Non-Histone/chemistry; Chromosomal Proteins, Non-Histone/genetics; Chromosomal Proteins, Non-Histone/metabolism; Chromosome Breakage/genetics; Chromosome Segregation; Chromosomes/chemistry; Chromosomes/genetics; Chromosomes/metabolism; Drosophila Proteins; Drosophila melanogaster/cytology; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Embryo, Nonmammalian/embryology; Embryo, Nonmammalian/metabolism; Insect Proteins/chemistry; Insect Proteins/genetics; Insect Proteins/metabolism; Mitosis/genetics; Molecular Weight; Muscle Proteins/chemistry; Muscle Proteins/genetics; Muscle Proteins/metabolism; Muscles/chemistry; Muscles/cytology; Muscles/embryology; Muscles/metabolism; Mutation/genetics; Phenotype; Protein Kinases/chemistry; Protein Kinases/genetics; Protein Kinases/metabolism; Sarcomeres/chemistry; Sarcomeres/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
