GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:11038171
| Citation |
Koch, M, Murrell, JR, Hunter, DD, Olson, PF, Jin, W, Keene, DR, Brunken, WJ and Burgeson, RE (2000) A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin: identification, expression, and functional characterization. J. Cell Biol. 151:221-34 |
|---|---|
| Abstract |
The netrins are a family of laminin-related molecules. Here, we characterize a new member of the family, beta-netrin. beta-Netrin is homologous to the NH(2) terminus of laminin chain short arms; it contains a laminin-like domain VI and 3.5 laminin EGF repeats and a netrin C domain. Unlike other netrins, this new netrin is more related to the laminin beta chains, thus, its name beta-netrin. An initial analysis of the tissue distribution revealed that kidney, heart, ovary, retina, and the olfactory bulb were tissues of high expression. We have expressed the molecule in a eukaryotic cell expression system and made antibodies to the expressed product. Both in situ hybridization and immunohistochemistry were used to describe the cellular source of beta-netrin and where beta-netrin is deposited. beta-Netrin is a basement membrane component; it is present in the basement membranes of the vasculature, kidney, and ovaries. In addition, beta-netrin is expressed in a limited set of fiber tracts within the brain, including the lateral olfactory tract and the vomeronasal nerve. Functional studies were performed and show that beta-netrin promotes neurite elongation from olfactory bulb explants. Together, these data suggest that beta-netrin is important in neural, kidney, and vascular development. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Basement Membrane/chemistry; Female; Genitalia, Female/chemistry; Humans; Kidney/chemistry; Laminin; Membrane Proteins/genetics; Membrane Proteins/immunology; Membrane Proteins/isolation & purification; Membrane Proteins/pharmacology; Mice; Molecular Sequence Data; Multigene Family; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/immunology; Nerve Tissue Proteins/isolation & purification; Nerve Tissue Proteins/pharmacology; Neurites; Olfactory Bulb/chemistry; Olfactory Bulb/drug effects; Rats; Recombinant Proteins/immunology; Recombinant Proteins/isolation & purification; Sequence Homology, Amino Acid; Tissue Distribution |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
