PMID:10948150

Citation	Householder, TC, Fozo, EM, Cardinale, JA and Clark, VL (2000) Gonococcal nitric oxide reductase is encoded by a single gene, norB, which is required for anaerobic growth and is induced by nitric oxide. Infect. Immun. 68:5241-6
Abstract	The gene encoding a nitric oxide reductase has been identified in Neisseria gonorrhoeae. The norB gene product shares significant identity with the nitric oxide reductases in Ralstonia eutropha and Synechocystis sp. and, like those organisms, the gonococcus lacks a norC homolog. The gonococcal norB gene was found to be required for anaerobic growth, but the absence of norB did not dramatically decrease anaerobic survival. In a wild-type background, induction of norB expression was seen anaerobically in the presence of nitrite but not anaerobically without nitrite or aerobically. norB expression is not regulated by FNR or NarP, but a functional aniA gene (which encodes an anaerobically induced outer membrane nitrite reductase) is necessary for expression. When aniA is constitutively expressed, norB expression can be induced both anaerobically and aerobically, but only in the presence of nitrite, suggesting that nitric oxide, which is likely to be produced by AniA as a product of nitrite reduction, is the inducing agent. This was confirmed with the use of the nitric oxide donor, spermine-nitric oxide complex, in an aniA null background both anaerobically and aerobically. NorB is important for gonococcal adaptation to an anaerobic environment, a physiologically relevant state during gonococcal infection. The presence of this enzyme, which is induced by nitric oxide, may also have implications in immune evasion and immunomodulation in the human host.
Links	PubMed PMC101784
Keywords	Amino Acid Sequence; Anaerobiosis; Antigens, Bacterial/physiology; Bacterial Outer Membrane Proteins/physiology; Bacterial Proteins/physiology; Base Sequence; DNA-Binding Proteins/physiology; Escherichia coli Proteins; Genes, Bacterial; Humans; Molecular Sequence Data; Neisseria gonorrhoeae/genetics; Neisseria gonorrhoeae/growth & development; Nitric Oxide/physiology; Oxidoreductases/genetics; Oxidoreductases/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NEIG2:B4RMX9		GO:0016966: nitric oxide reductase activity	ECO:0000247:	UniProtKB:O30375 UniProtKB:P74677 UniProtKB:Q7WX97	F		Sequence alignment shown in FIGURE 1	complete CACAO 2484
NEIG2:B4RMX9	enables	GO:0016966: nitric oxide reductase activity	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P74677	F		Seeded From UniProt	complete

References

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