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PMID:10799297
| Citation |
Wyss, A, Wirtz, G, Woggon, W, Brugger, R, Wyss, M, Friedlein, A, Bachmann, H and Hunziker, W (2000) Cloning and expression of beta,beta-carotene 15,15'-dioxygenase. Biochem. Biophys. Res. Commun. 271:334-6 |
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| Abstract |
beta,beta-Carotene 15,15'-dioxygenase cleaves beta-carotene into two molecules of retinal and is therefore the key enzyme in beta-carotene metabolism to vitamin A. In the present study, it was possible to enrich the chicken beta,beta-carotene 15,15'-dioxygenase to such an extent that partial amino acid sequence information could be obtained to design degenerate oligonucleotides. With RT-PCR a cDNA fragment could be obtained and used subsequently in a radioactive screening of a chicken duodenal expression library. We cloned the first eukaryotic beta,beta-carotene 15,15'-dioxygenase which symmetrically cleaves beta-carotene at the 15,15'-double bond. |
| Links |
PubMed Online version:10.1006/bbrc.2000.2619 |
| Keywords |
Animals; Cell Line; Chickens; Chromatography, High Pressure Liquid; Cloning, Molecular; Cricetinae; Cytoplasm/enzymology; DNA, Complementary/metabolism; Duodenum/enzymology; Electrophoresis, Polyacrylamide Gel; Oxygenases/chemistry; Oxygenases/genetics; Oxygenases/metabolism; Peptides/chemistry; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; beta-Carotene 15,15'-Monooxygenase |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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