PMID:10747038

Citation	Oda, S, Humbert, O, Fiumicino, S, Bignami, M and Karran, P (2000) Efficient repair of A/C mismatches in mouse cells deficient in long-patch mismatch repair. EMBO J. 19:1711-8
Abstract	A previously unrecognized mismatch repair activity is described. Extracts of immortalized MSH2-deficient mouse fibroblasts did not correct most single base mispairs. The same extracts carried out efficient repair of A/C mismatches. A/G mispairs were less efficiently corrected and there was no significant repair of A/A. MLH1-defective mouse extracts also repaired an A/C mispair. A/C correction by Msh2(-/-) mouse cell extracts was not affected by antibodies against the PMS2 protein, which inhibited long-patch mismatch repair. A/C repair activity is thus independent of MutSalpha, MutSbeta and MutLalpha. A/C mismatches were corrected 5-fold more efficiently by extracts of Msh2 knockout mouse cells than by comparable extracts prepared from hMSH2- or hMLH1-deficient human cells. MSH2-independent A/C correction by mouse cell extracts did not require a nick in the circular duplex DNA substrate. Repair involved replacement of the A and was associated with the resynthesis of a limited stretch of </=25 bases of DNA.
Links	PubMed PMC310239 Online version:10.1093/emboj/19.7.1711
Keywords	Adaptor Proteins, Signal Transducing; Animals; Base Pair Mismatch; Base Sequence; Carrier Proteins; Cell Line; DNA Primers/genetics; DNA Repair; DNA-Binding Proteins; HeLa Cells; Humans; Mice; Mice, Knockout; MutS Homolog 2 Protein; Neoplasm Proteins/genetics; Neoplasm Proteins/metabolism; Nuclear Proteins; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/metabolism
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